5NKS
Human Dihydropyrimidinase-related Protein 4 (DPYSL4, CRMP3, ULIP-4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.470, 102.650, 116.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.160 - 1.800 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.20049 |
| Starting model (for MR) | 4bkn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.831 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.850 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.651 |
| Number of reflections | 44328 | 2548 |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 98.3 | 96.6 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M Bis-tris-propane 0.2 M Sodium fluoride 10% Ethylene glycol 20% PEG3350 |
