5NJY
X-ray structure of the H235Q mutant of GLIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-05-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.925, 134.239, 160.628 |
| Unit cell angles | 90.00, 102.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 77705 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 97.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 400 mM NaSCN 100 mM Na Acetate pH 4 12-15% PEG4000 2% DMSO 16% Glycerol |
