5NJM

Lysozyme room-temperature structure determined by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-05-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	78.550, 78.550, 38.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.638 - 1.500
R-factor	0.1585
Rwork	0.156
R-free	0.19950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lzt
RMSD bond length	0.009
RMSD bond angle	0.946
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.800	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	20181	1029
<I/σ(I)>	7.51	0.72
Completeness [%]	94.7	49.23
Redundancy	452.9	4.6
CC(1/2)	0.996	0.169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	293	19.04% NaCl, 5.44% PEG 8,000, 68mM Na Acetate pH 3.0