5NIZ
EthR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9600 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.477, 120.477, 33.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.910 - 1.950 |
| R-factor | 0.17792 |
| Rwork | 0.175 |
| R-free | 0.22928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.618 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.910 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 18652 |
| <I/σ(I)> | 13.6 |
| Completeness [%] | 99.8 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MEs pH 6-6.5, 1.4-1.6 M AS |
