Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.044, 302.010, 143.840 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 50.010 - 3.000 |
| R-factor | 0.1769 |
| Rwork | 0.174 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.197 | |
| Number of reflections | 210084 | 20926 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M MES pH 6.5, 35mM magnesium acetate, 13% MPD |
