5NGW

Glycoside hydrolase-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	102.054, 102.054, 80.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	88.380 - 2.400
R-factor	0.16644
Rwork	0.164
R-free	0.21468
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4acy
RMSD bond length	0.015
RMSD bond angle	1.653
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.380	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.112	0.741
Rpim		0.250
Number of reflections	19223	2761
<I/σ(I)>	13.6	3
Completeness [%]	100.0	100
Redundancy	9.6	9.8
CC(1/2)	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	100 mM Tris pH 8.0 20 % PEG 3350