5NGO
Crystal structure of the PARP domain of Arabidopsis RADICAL-INDUCED CELL DEATH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 129.952, 129.952, 468.376 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 156.130 - 2.500 |
| R-factor | 0.2371 |
| Rwork | 0.236 |
| R-free | 0.26340 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 156.130 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.117 | 1.019 |
| Number of reflections | 52927 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 30.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 1.6M Ammoniumsulfate, 2% PEG1000 |
