5NGJ
Crystal structure of pb6, major tail tube protein of bacteriophage T5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.790, 115.081, 83.366 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.540 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 68061 |
| <I/σ(I)> | 14.4 |
| Completeness [%] | 99.7 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 10 % 3350 PEG, 0.2 M HEPES pH 7.5, 0.3 M LiSO4, 0.1 mM MgCl2 |
