5NG4
Human CEP135 parallel dimeric coiled coil 82-144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.0664 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 79.000, 79.000, 95.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.500 - 2.140 |
| R-factor | 0.2267 |
| Rwork | 0.223 |
| R-free | 0.25770 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.310 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.210 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.089 | 0.178 |
| Number of reflections | 7467 | 356 |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 86.0 | 43 |
| Redundancy | 41.5 | 6.2 |
| CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 25% PEG 1500, SPG buffer, pH 6.0 |
