5NFD

Antiparallel monomeric coiled coil of Kif21A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	2.0664
Spacegroup name	P 21 21 21
Unit cell lengths	33.970, 41.670, 123.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.498 - 2.180
R-factor	0.2015
Rwork	0.197
R-free	0.23920
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.426
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX (dev_1965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.680	2.250
High resolution limit [Å]	2.180	2.180
Rmerge	0.031	0.074
Number of reflections	8779
<I/σ(I)>	54.6	9.6
Completeness [%]	90.0	35
Redundancy	9.6	2.1
CC(1/2)	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES, pH 7.0, 20% isopropanol