5NF4

The tip fimbrial protein Mfa3 from Porphyromonas gingivalis with C-terminal truncation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97623
Spacegroup name	P 32
Unit cell lengths	80.183, 80.183, 116.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.520 - 1.746
R-factor	0.1506
Rwork	0.148
R-free	0.17730
RMSD bond length	0.010
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.441	44.520	1.840
High resolution limit [Å]	1.721	5.520	1.750
Rmerge		0.033	0.365
Rmeas	0.043	0.044	0.502
Rpim	0.028	0.029	0.343
Total number of observations	134723
Number of reflections	79050
<I/σ(I)>	12.2	16.2	2.1
Completeness [%]	93.2	84.2	93.4
Redundancy	1.7	1.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	3% PGA, 8% PEG 8000, 0.3 M sodium formate, 0.12 M ammonium sulfate and 0.1 M sodium acetate, pH 5.0.