5NF3
The fimbrial shaft protein Mfa1 from Porphyromonas gingivalis-C-terminal deletion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.262, 66.262, 286.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.648 - 1.970 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.650 | 48.650 | 2.040 |
| High resolution limit [Å] | 1.970 | 7.630 | 1.970 |
| Rmerge | 0.211 | 0.063 | 1.739 |
| Rmeas | 0.220 | 0.066 | 1.809 |
| Rpim | 0.061 | 0.020 | 0.495 |
| Total number of observations | 587519 | ||
| Number of reflections | 46649 | ||
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 12.6 | 10 | 13.2 |
| CC(1/2) | 0.997 | 0.999 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 8000, 75 mM calcium acetate, 0.1 M sodium cacodylate pH 6.5. |
