5NF3

The fimbrial shaft protein Mfa1 from Porphyromonas gingivalis-C-terminal deletion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	66.262, 66.262, 286.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.648 - 1.970
R-factor	0.1804
Rwork	0.179
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650	48.650	2.040
High resolution limit [Å]	1.970	7.630	1.970
Rmerge	0.211	0.063	1.739
Rmeas	0.220	0.066	1.809
Rpim	0.061	0.020	0.495
Total number of observations	587519
Number of reflections	46649
<I/σ(I)>	14.9
Completeness [%]	100.0	99.7	100
Redundancy	12.6	10	13.2
CC(1/2)	0.997	0.999	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG 8000, 75 mM calcium acetate, 0.1 M sodium cacodylate pH 6.5.