5NF2
The fimbrial shaft protein Mfa1 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.846, 65.846, 284.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.297 - 1.730 |
| R-factor | 0.1534 |
| Rwork | 0.152 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | SCALA (0.5.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.300 | 48.300 | 1.790 |
| High resolution limit [Å] | 1.730 | 6.700 | 1.730 |
| Rmerge | 0.079 | 0.038 | 1.191 |
| Rmeas | 0.085 | 0.042 | 1.280 |
| Rpim | 0.032 | 0.017 | 0.465 |
| Total number of observations | 478679 | ||
| Number of reflections | 66708 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 99.9 | 99.7 | 99.8 |
| Redundancy | 7.2 | 5.8 | 7.4 |
| CC(1/2) | 0.999 | 0.998 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 8000, 75 mM calcium acetate, 0.1 M sodium cacodylate pH 6.5. |
