5NF1

crystal structure of variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	83.198, 62.783, 136.259
Unit cell angles	90.00, 96.35, 90.00

Refinement procedure

Resolution	47.962 - 2.697
R-factor	0.2361
Rwork	0.232
R-free	0.32120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.072
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.962
High resolution limit [Å]	2.697
Rmeas		0.624
Rpim	0.057	0.346
Number of reflections	35020
<I/σ(I)>	10.18
Completeness [%]	97.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	PEG Tris cl