5NEW
RNA-RNA base stacking in the crystal structure of an Hfq6:RNA dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979681 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 66.880, 66.880, 227.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.600 - 2.511 |
| R-factor | 0.1993 |
| Rwork | 0.196 |
| R-free | 0.25220 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.600 |
| High resolution limit [Å] | 2.510 |
| Number of reflections | 6816 |
| <I/σ(I)> | 15.03 |
| Completeness [%] | 96.8 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M phosphate-citrate buffer pH 4.2, 27% PEG 1000, and 0.2 M LiSO4 |
