5NEO
The structure of the G. violaceus guanidine II riboswitch P1 stem-loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92011 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.302, 55.302, 130.164 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.651 - 1.690 |
| R-factor | 0.2166 |
| Rwork | 0.216 |
| R-free | 0.23030 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.890 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.066 | 1.500 |
| Rpim | 0.016 | 0.432 |
| Number of reflections | 16551 | 422 |
| <I/σ(I)> | 17.1 | 1.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 19.04 | 18.5 |
| CC(1/2) | 0.950 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.01 M Magnesium Acetate, 0.05 M MES pH 5.6, 2.5 M Ammonium Sulfate |
