5NDK

Crystal structure of aminoglycoside TC007 co-crystallized with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2016-05-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	209.070, 447.360, 619.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.739 - 2.950
R-factor	0.2135
Rwork	0.213
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.915
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.739	300.000	3.050
High resolution limit [Å]	2.950	200.000	2.950
Rmerge	0.307		2.574
Rmeas	0.310		2.636
Total number of observations	56386821
Number of reflections	1204009		113823
<I/σ(I)>	16.04		1.38
Completeness [%]	100.0		100
Redundancy	46.833		21.11
CC(1/2)	0.994		0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN