5NC0
The 0.91 A resolution structure of the L16G mutant of cytochrome c prime from Alcaligenes xylosoxidans, complexed with nitric oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.78720 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.430, 52.430, 183.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.070 - 0.910 |
| R-factor | 0.1249 |
| Rwork | 0.125 |
| R-free | 0.13177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.471 |
| Data reduction software | XDS (20161101) |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | REFMAC (5.8.0158) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.070 | 0.930 |
| High resolution limit [Å] | 0.910 | 0.910 |
| Rpim | 0.023 | 0.564 |
| Number of reflections | 106845 | 4889 |
| <I/σ(I)> | 16.1 | 1.3 |
| Completeness [%] | 99.5 | 93.9 |
| Redundancy | 8 | 4.4 |
| CC(1/2) | 0.999 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | AMMONIUM SULPHATE, TRIS PH 7.5 |
