5NBL
Crystal structure of the Arp4-N-actin(APO-state) heterodimer bound by a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 191.224, 191.224, 221.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.426 - 2.800 |
| R-factor | 0.1727 |
| Rwork | 0.171 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yag 3qb0 nanobody homology model |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.702 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.430 | 49.430 | 2.850 |
| High resolution limit [Å] | 2.800 | 15.340 | 2.800 |
| Rmerge | 0.156 | 0.057 | 1.092 |
| Rmeas | 0.170 | 0.062 | 1.217 |
| Rpim | 0.068 | 0.025 | 0.531 |
| Total number of observations | 660887 | ||
| Number of reflections | 112494 | ||
| <I/σ(I)> | 10.4 | 24.6 | 1.9 |
| Completeness [%] | 99.7 | 95.3 | 99.9 |
| Redundancy | 5.9 | 5.7 | 5 |
| CC(1/2) | 0.995 | 0.997 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1 mM ADP, 0.2 mM CaCl2, 1.3-1.5 M sodium malonate at pH 6.0, Subtilisin (1/6000; w(subtilisin)/w(protein)) |
