5NB4
Atomic resolution structure of C-phycoerythrin from marine cyanobacterium Phormidium sp. A09DM at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 |
| Unit cell lengths | 109.046, 109.095, 117.371 |
| Unit cell angles | 78.78, 82.32, 60.26 |
Refinement procedure
| Resolution | 94.620 - 1.140 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.18400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5aqd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.620 | 1.160 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.042 | 0.545 |
| Rpim | 0.042 | 0.545 |
| Number of reflections | 1575226 | 74960 |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 93.8 | 90 |
| Redundancy | 2.6 | 2.7 |
| CC(1/2) | 0.998 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | Morpheus screen condition C8 with the NPS mix of additives |
