5NB3
High resolution C-phycoerythrin from marine cyanobacterium Phormidium sp. A09DM at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92819 |
Spacegroup name | P 1 |
Unit cell lengths | 110.046, 110.170, 118.516 |
Unit cell angles | 78.76, 82.28, 60.43 |
Refinement procedure
Resolution | 95.420 - 1.380 |
R-factor | 0.158 |
Rwork | 0.155 |
R-free | 0.21100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5aqd |
RMSD bond length | 0.018 |
RMSD bond angle | 2.596 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.420 | 1.420 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.077 | 0.801 |
Rpim | 0.077 | 0.801 |
Number of reflections | 932250 | 67249 |
<I/σ(I)> | 7.3 | 1.3 |
Completeness [%] | 95.7 | 93.2 |
Redundancy | 2.9 | 2.8 |
CC(1/2) | 0.997 | 0.477 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | Morpheus screen condition G8 |