5NB3

High resolution C-phycoerythrin from marine cyanobacterium Phormidium sp. A09DM at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92819
Spacegroup name	P 1
Unit cell lengths	110.046, 110.170, 118.516
Unit cell angles	78.76, 82.28, 60.43

Refinement procedure

Resolution	95.420 - 1.380
R-factor	0.158
Rwork	0.155
R-free	0.21100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5aqd
RMSD bond length	0.018
RMSD bond angle	2.596
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.420	1.420
High resolution limit [Å]	1.380	1.380
Rmerge	0.077	0.801
Rpim	0.077	0.801
Number of reflections	932250	67249
<I/σ(I)>	7.3	1.3
Completeness [%]	95.7	93.2
Redundancy	2.9	2.8
CC(1/2)	0.997	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294.15	Morpheus screen condition G8