5NAZ

Crystal structures of homooligomers of collagen type IV. alpha5NC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.98
Spacegroup name	P 41 3 2
Unit cell lengths	121.283, 121.283, 121.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.870 - 1.850
R-factor	0.1855
Rwork	0.185
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t60
RMSD bond length	0.009
RMSD bond angle	1.246
Data reduction software	XDS
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.880	5.850	1.950
High resolution limit [Å]	1.850	4.140	1.850
Rmerge	0.100	0.047	0.354
Rmeas	0.112	0.053	0.396
Rpim	0.049	0.023	0.174
Total number of observations	125648
Number of reflections	26393
<I/σ(I)>	12.1	11.7	2
Completeness [%]	99.3	96.4	99.9
Redundancy	4.8	4.6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	20%PEG8000, 0.2 M NaCl, CAPS pH 10.5