5N9E
Crystal Structure of Drosophila DHX36 helicase in complex with TGGGGATTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 304.472, 51.242, 164.522 |
| Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
| Resolution | 56.620 - 3.007 |
| R-factor | 0.1853 |
| Rwork | 0.182 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n8r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2427: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.620 | 3.114 |
| High resolution limit [Å] | 3.007 | 3.007 |
| Rmerge | 0.109 | 0.670 |
| Number of reflections | 44068 | 4576 |
| <I/σ(I)> | 10.92 | 2.16 |
| Completeness [%] | 93.9 | 99.85 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.994 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate |
