5N96
Crystal Structure of Drosophila DHX36 helicase in complex with AGGGTTTTTT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 303.389, 51.316, 164.602 |
Unit cell angles | 90.00, 114.57, 90.00 |
Refinement procedure
Resolution | 56.650 - 2.716 |
R-factor | 0.1832 |
Rwork | 0.180 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n8r |
RMSD bond length | 0.011 |
RMSD bond angle | 1.409 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2427: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.650 | 2.813 |
High resolution limit [Å] | 2.716 | 2.716 |
Rmerge | 0.102 | 0.721 |
Number of reflections | 61776 | 6222 |
<I/σ(I)> | 10.9 | 2.28 |
Completeness [%] | 97.6 | 99.86 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.995 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate |