5N90

Crystal Structure of Drosophila DHX36 helicase in complex with TTGTGGTGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	C 1 2 1
Unit cell lengths	307.025, 50.993, 165.266
Unit cell angles	90.00, 115.38, 90.00

Refinement procedure

Resolution	74.660 - 3.069
R-factor	0.1889
Rwork	0.186
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n8r
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.660	3.179
High resolution limit [Å]	3.069	3.069
Rmerge	0.117	0.833
Number of reflections	44213	4405
<I/σ(I)>	14.28	2.34
Completeness [%]	99.9	99.93
Redundancy	6.7	6.4
CC(1/2)	0.998	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate