5N8W
CRYSTAL STRUCTURE OF STREPTAVIDIN with D-amino acid containing peptide GGwhdeatwkpG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.882, 95.720, 82.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.300 - 1.100 |
| R-factor | 0.13599 |
| Rwork | 0.135 |
| R-free | 0.15093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.300 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.048 | 0.400 |
| Number of reflections | 122566 | 28666 |
| <I/σ(I)> | 17.4 | 4.01 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 6.2 | 5.29 |
| CC(1/2) | 0.990 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Magnesium acetate 0.1M MOPS pH 7.5 12% PEG8000 |
