5N8S
Crystal Structure of Drosophila DHX36 helicase in complex with polyT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9754 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 297.981, 49.923, 163.847 |
| Unit cell angles | 90.00, 115.01, 90.00 |
Refinement procedure
| Resolution | 81.100 - 2.880 |
| R-factor | 0.2112 |
| Rwork | 0.207 |
| R-free | 0.28470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n8r |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.781 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.100 | 2.983 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.098 | 0.489 |
| Number of reflections | 50207 | 4979 |
| <I/σ(I)> | 8.91 | 2.24 |
| Completeness [%] | 99.4 | 99.82 |
| Redundancy | 3.3 | 3.5 |
| CC(1/2) | 0.991 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate |
