5N8D
Structure of the distal domain of mouse adenovirus 2 fibre, P21 native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | Dectris |
| Wavelength(s) | 1.0023 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.880, 100.600, 95.470 |
| Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
| Resolution | 29.660 - 1.800 |
| R-factor | 0.16889 |
| Rwork | 0.168 |
| R-free | 0.20082 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n83 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.588 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.660 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.334 |
| Number of reflections | 144435 | 7096 |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 2.8 | 2.9 |
| CC(1/2) | 0.989 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 10 mM Tris-HCl, 4.0 M ammonium formate, 0.1 M HEPES-NaOH |
