5N83
Structure of the distal domain of mouse adenovirus 2 fibre, methylmercury chloride derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0024 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 92.116, 164.285, 96.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.140 - 2.760 |
| R-factor | 0.20507 |
| Rwork | 0.203 |
| R-free | 0.24824 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.400 | 3.020 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.104 | 0.589 |
| Number of reflections | 19199 | 4473 |
| <I/σ(I)> | 19.6 | 3.2 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 11.7 | 8 |
| CC(1/2) | 0.998 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 4.0 M ammonium formate, 0.1 M HEPES-NaOH, 3 mM methylmercury chloride, 10 mM Tris-HCl |
