5N80

glycosyltransferase LPS biosynthesis in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	77
Detector technology	CCD
Collection date	2015-05-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	104.440, 104.440, 89.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.850 - 1.920
R-factor	0.241
Rwork	0.239
R-free	0.27100
RMSD bond length	0.008
RMSD bond angle	0.915
Data scaling software	xia2
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	73.850
High resolution limit [Å]	1.920
Number of reflections	38460
<I/σ(I)>	1.9
Completeness [%]	99.5
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	1% trypton; 0.001M sodium azide; 0.05M HEPES pH7.0 and 20% w/v PEG 3350