5N7Z

glycosyltransferase in LPS biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	77
Detector technology	CCD
Collection date	2015-05-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.8233
Spacegroup name	P 43 21 2
Unit cell lengths	104.270, 104.270, 88.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.446 - 1.810
R-factor	0.2309
Rwork	0.229
R-free	0.25910
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.000
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	67.640
High resolution limit [Å]	1.810
Number of reflections	44340
<I/σ(I)>	3.1
Completeness [%]	98.5
Redundancy	18.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298.15	1% trypton; 0.001M sodium azide; 0.05M HEPES pH7.0 and 20% w/v PEG 3350