5N7K
Crystal structure of the coiled-coil domain of human tricellulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.621, 82.621, 188.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.260 - 2.810 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n7h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 2.906 |
| High resolution limit [Å] | 2.805 | 2.805 |
| Rmerge | 0.086 | 0.975 |
| Number of reflections | 18866 | 1787 |
| <I/σ(I)> | 18.15 | 2.04 |
| Completeness [%] | 99.4 | 97.01 |
| Redundancy | 10.2 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 55% (v/v) MPD, 0.1 M Mes pH 5.0 |
