5N7H
Crystal structure of the coiled-coil domain of human tricellulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 44.039, 44.039, 202.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.140 - 2.200 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.25800 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.039 | 2.276 |
| High resolution limit [Å] | 2.198 | 2.198 |
| Rmerge | 0.046 | 0.887 |
| Number of reflections | 6591 | 616 |
| <I/σ(I)> | 33.69 | 3.3 |
| Completeness [%] | 99.7 | 99.52 |
| Redundancy | 12.7 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% (v/v) isopropanol, 0.1 M citrate pH 5.0 |
