5N7G
MAGI-1 complexed with a synthetic pRSK1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.930, 60.230, 99.350 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 49.105 - 2.950 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xjl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.499 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.105 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmeas | 0.268 | 0.897 |
| Number of reflections | 24379 | |
| <I/σ(I)> | 8.36 | 2.49 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.86 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 14% PEG 8000, 200 mM MgCl2, 100 mM TRIS |
