5N7F
MAGI-1 complexed with a pRSK1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.150, 98.560, 200.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.730 - 2.300 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xjl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.871 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.730 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.219 | |
| Number of reflections | 53922 | |
| <I/σ(I)> | 15.42 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.87 | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 14% PEG 8000, 200 mM MgCl2, 100 mM TRIS |
