5N72
Crystal structure of the Legionella effector WipA shorter construct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.060, 57.480, 80.710 |
| Unit cell angles | 90.00, 91.15, 90.00 |
Refinement procedure
| Resolution | 43.034 - 1.842 |
| R-factor | 0.2211 |
| Rwork | 0.219 |
| R-free | 0.25710 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.690 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rpim | 0.039 | |
| Number of reflections | 38861 | |
| <I/σ(I)> | 16.2 | 1.58 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M sodium acetate and 18% PEG3350 |
