5N6B
Human Leukocyte Antigen Class I A02 Carrying LLWNPGMAV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.090, 168.990, 49.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.800 - 1.710 |
| R-factor | 0.18423 |
| Rwork | 0.183 |
| R-free | 0.21448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eu5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.800 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.094 | |
| Rmeas | 0.094 | 1.335 |
| Rpim | 0.055 | |
| Number of reflections | 107271 | |
| <I/σ(I)> | 11.9 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | |
| CC(1/2) | 0.998 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M ammonium sulphate, 0.1M HEPES pH7, 20% PEG 4000 |
