5N5P
Crystal structure of Ruminococcus flavefaciens' type III complex containing the fifth cohesin from scaffoldin B and the dockerin from scaffoldin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.82655 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.093, 142.898, 46.588 |
Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
Resolution | 46.580 - 1.980 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lxv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.390 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.630 | 47.630 | 2.050 |
High resolution limit [Å] | 1.400 | 8.940 | 2.000 |
Rmerge | 0.098 | 0.064 | 0.294 |
Rmeas | 0.106 | 0.069 | 0.318 |
Rpim | 0.040 | 0.026 | 0.120 |
Number of reflections | 26512 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 100.0 | 99.7 | 99.8 |
Redundancy | 6.9 | 6.7 | 6.9 |
CC(1/2) | 0.995 | 0.997 | 0.974 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1M HEPES 7.5, 1.2M Sodium Citrate, 4% v/v Acetonitrile |