5N5F

Crystal structure of Haliangium ochraceum encapsulated ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.72
Spacegroup name	C 1 2 1
Unit cell lengths	91.629, 92.652, 119.291
Unit cell angles	90.00, 106.94, 90.00

Refinement procedure

Resolution	47.820 - 2.057
R-factor	0.2011
Rwork	0.199
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5da5
RMSD bond length	0.003
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	2.131
High resolution limit [Å]	2.057	2.057
Rmerge	0.094	0.597
Number of reflections	57293	5570
<I/σ(I)>	10.16	2.37
Completeness [%]	96.0	94
Redundancy	5.2	4.9
CC(1/2)	0.997	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.2 M NaCl, 0.1 M Bis-Tris.HCl pH 5.5, 20 % (w/v) PEG3350