5N4M

Human myelin protein P2, mutant I43N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	64.997, 64.997, 101.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.960 - 1.590
R-factor	0.1857
Rwork	0.184
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bvm
RMSD bond length	0.007
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	1.690
High resolution limit [Å]	1.590	1.590
Rmeas	0.070	3.270
Number of reflections	29518
<I/σ(I)>	18.4	0.7
Completeness [%]	98.7	89.2
Redundancy	6.6	6.4
CC(1/2)	1.000	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.1 M DL-malic acid