5N4K
N-terminal domain of a human Coronavirus NL63 nucleocapsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 32 |
| Unit cell lengths | 48.090, 48.090, 101.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.650 - 1.490 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxx |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.533 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.610 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.034 | 0.095 |
| Number of reflections | 42346 | |
| <I/σ(I)> | 16.1 | 7.4 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | AMMONIUM SULPHATE, TRIS, PH 8.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
