5N2U
Influenza D virus nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.166, 85.166, 103.385 |
| Unit cell angles | 91.20, 101.94, 101.02 |
Refinement procedure
| Resolution | 39.710 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zdp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.440 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.400 |
| Number of reflections | 93538 | 8505 |
| <I/σ(I)> | 7.78 | 1.05 |
| Completeness [%] | 91.3 | 94.1 |
| Redundancy | 2.2 | 2.2 |
| CC(1/2) | 0.994 | 0.366 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M sodium malonate pH 4 |
