5N2P

Sulfolobus solfataricus Tryptophan Synthase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-01
Detector	MAR CCD 130 mm
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	76.540, 76.540, 122.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.270 - 2.059
R-factor	0.2274
Rwork	0.226
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wxj
RMSD bond length	0.005
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.270	2.130
High resolution limit [Å]	2.059	2.059
Rmerge		0.068
Rmeas	0.068
Number of reflections	22885	1984
<I/σ(I)>	30.76	2.99
Completeness [%]	98.0	99
Redundancy	18.9	7.5
CC(1/2)		0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8.0, 20% [v/v] PEG 300, 5% [w/v] PEG 8000, 10% Glycerol