5N1T
Crystal structure of complex between flavocytochrome c and copper chaperone CopC from T. paradoxus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976256 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.530, 138.400, 155.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.18949 |
| Rwork | 0.186 |
| R-free | 0.25703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fcd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.947 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.117 | 0.910 |
| Number of reflections | 28526 | 2841 |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 93.1 | 99.8 |
| Redundancy | 3.7 | 5.5 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 0.056 M Sodium phosphate monobasic monohydrate, 1.344 M Potassium phosphate dibasic |
