5N0K
Rat ceruloplasmin orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.360, 103.810, 172.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.300 |
| R-factor | 0.17706 |
| Rwork | 0.175 |
| R-free | 0.22627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4enz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.838 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.180 | 1.389 |
| Rmeas | 0.186 | 1.437 |
| Rpim | 0.048 | 0.367 |
| Number of reflections | 61072 | |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG8000, LITHIUM SULFATE, 0.1M TRIS |
