5MZO
UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (open conformation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91741 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 163.570, 163.570, 248.584 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 102.000 - 3.480 |
| R-factor | 0.252 |
| Rwork | 0.251 |
| R-free | 0.27800 |
| Starting model (for MR) | 5mu1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.290 | 3.680 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.250 | 3.180 |
| Rpim | 0.100 | 1.290 |
| Number of reflections | 25652 | 3643 |
| <I/σ(I)> | 9.2 | 0.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11.8 | 12.2 |
| CC(1/2) | 0.997 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 24 mM Morpheus alchools mix, 20 mM Morpheus buffer system 3 pH 8.5, 2.5% v/v MPD, 2.5% w/v PEG 1000, 2.5% w/v PEG 3350, 1.6 mM CaCl2 and 50 uM UDP-glucose |
