5MZN
Helicase Sen1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.285, 171.944, 69.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.390 - 1.787 |
| Rwork | 0.153 |
| R-free | 0.18360 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 1.851 |
| High resolution limit [Å] | 1.787 | 1.787 |
| Rmerge | 0.085 | 1.500 |
| Number of reflections | 100766 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 98.3 | 95.07 |
| Redundancy | 13.2 | 13.5 |
| CC(1/2) | 0.999 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 18% PEG 2K 0.1M MOPS 7.2 |
