5MZH
Crystal structure of ODA16 from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.767, 62.767, 460.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.154 - 2.398 |
| R-factor | 0.1609 |
| Rwork | 0.158 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wjs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.125 | 0.884 |
| Number of reflections | 39781 | |
| <I/σ(I)> | 7.3 | 0.8 |
| Completeness [%] | 99.7 | 82 |
| Redundancy | 6.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 300 mM ammonium sulfate and 100 mM Bis-Tris pH 5.5 |
