5MY7
Adhesin Complex Protein from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.470, 58.470, 51.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.640 - 1.400 |
| R-factor | 0.16191 |
| Rwork | 0.160 |
| R-free | 0.20045 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.776 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0157) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.640 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.054 | 0.997 |
| Rpim | 0.036 | 0.668 |
| Number of reflections | 20461 | 1001 |
| <I/σ(I)> | 13.5 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 25 mM Hepes/Na pH 7.5, 37 mM NaCl) with 200 nl precipitant solution containing 0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5 and 50 % w/v PEG 400 |
