5MXZ
Kustc0563 Y40F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.520, 47.520, 99.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.900 - 1.900 |
| R-factor | 0.20187 |
| Rwork | 0.201 |
| R-free | 0.22539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wt structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.241 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.800 |
| Rmeas | 0.080 | 0.854 |
| Number of reflections | 11201 | 1627 |
| <I/σ(I)> | 15.7 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | |
| CC(1/2) | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Zn-acetate, 0.1 M Mes pH 6.0, 10% (w/v) PEG8000 |
